二氢二醇脱氢酶2在前列腺癌中的表达及意义

Expression of dihydrodiol dehydrogenase 2 in prostate cancer and its significance

目的 探讨二氢二醇脱氢酶 (DD)在前列腺癌中的表达及意义。 方法 以 β actin为内参基因 ,应用逆转录聚合酶链反应方法检测DD亚型之一DD2在前列腺癌组织 (11例 )及正常前列腺组织 (10例 )中的表达水平。应用密度扫描技术定量分析RT PCR产物电泳条带的密度。 结果 DD2mRNA在前列腺癌组织中表达明显增高 ,吸光度比值 0 .5 5 0～ 1.0 18,中位数 0 .72 6 ;在正常前列腺组织中的表达水平较低 ,吸光度比值 0 .2 4 8～ 0 .4 2 0 ,中位数 0 .333。癌与正常前列腺组织间DD2mRNA表达差异有显著性意义 (P <0 .0 0 1)。 结论 DD2mRNA在前列腺癌中的高表达提示DD可能在前列腺癌的发生发展中发挥重要作用。

Objective To study the expression of dihydrodiol dehydrogenase (DD) in prostate cancer and its significance. Methods With reference to expression of β-actin gene,the expression level of a human dihydrodiol dehydrogenase isoform (DD2) mRNA was examined in prostate cancer tissues (11 cases) and normal prostate tissues (10 cases) by reverse transcriptive-polymerase chain reaction(RT-PCR). Quantitative determination of relevant band densities was performed using densitometry-scanning techniques. Results Strong expression of DD2 mRNA was detected in prostate cancer tissures with absorbance in the range of 0.550 to 1.018 (median,0.726),and low expression of DD2 mRNA in normal prostate tissues with absorbance in the range of 0.248 to 0.420 (median,0.333). The difference of the expression of DD2 mRNA between cancer and normal prostate was significant ( P <0.001). Conclusions The high expression level of DD2 mRNA in prostate cancer indicates that DD may play an important role in prostate carcinogenesis.