L-精氨酸对自体移植猪小肠粘膜Na^+-K^+-ATP酶活力的影响

EFFECT OF L-ARGININE ON Na^+-K^+-ATPASE ACTIVITY IN MUCOSA OF AUTOGRAFTED INTESTINE IN PIGS

目的 :探讨 L-精氨酸对自体移植小肠粘膜 ATP酶的影响。方法 :在建立幼猪自体小肠移植模型的基础上 ,再灌注期间静脉滴注 L-精氨酸 1 5 0 mg/kg。观察再灌注 2 4、48及 72 h时肠粘膜 Na+ - K+ - ATP酶活力的变化。结果 :实验组、假手术组和对照组术后 48h,实验组术后 72 h肠粘膜 Na+ - K+ - ATP酶活性较正常对照值明显增高 ;假手术组术后 72 h较 2 4、48h,术后 48h较 2 4 h的肠粘膜 Na+ - K+ - ATP酶活性值显著增高。实验组与假手术组及对照组间各时相比较 ,无显著性差异。结论 :幼猪小肠冷缺血再灌注操作后肠粘膜ATP酶活性有不同程度的增高 ,但应用 L-精氨酸并未明显增加 ATP酶的活性。应用

Objective:To investigate the effect of L arginine on intestinal mucosal Na + K + ATPase activity after auto transplantation of intestine.Methods:On the basis of establishment of auto transptantation of intestine in pigs,L arginine (150 mg/kg body weight) was given intravenously during cold ischemia and reperfusion period and Na + K + ATPase activity was monitored 24 h,48 h and 72 h after reperfusion.Results:Compared with the normal value,at 48 and 72 h in experiment group,at 48 h in pseudo operative group and control group,intestinal mucosal Na + K + ATPase activities were significantly high.In pseudo operative group,Na + K + ATPase activity was significantly higher at 72 h than that at 24 h and 48 h,and at 48 h was sigificantly higher than that at 24 h.However,in comparison with pseudo operative and control groups,there was no significant difference of Na + K + ATPase activity in experimental group.Conclusion:After cold ischemia reperfusion,Na + K + ATPase activities of intestinal mucosa increased,but did not show significant increase after administration of L arginine.The role of L arginine in prevention of cold ischemia reperfusion injury of intestine should be re evaluated in the future.