测定乙型肝炎病毒表面抗原S/CO值的临床应用评价

Evaluation of the clinical shgnifhcane of S/CO ratio of HBsAg test in patients with HBV infection

目的 探讨检测乙型肝炎 (简称乙肝 )病毒表面抗原 (HBsAg)S/CO值的临床应用价值。方法 用美国雅培公司生产的AXSYM全自动微粒子酶免疫荧光系统及Murex和科华HBsAgELISA试剂盒检测不同浓度HBsAg标准品的S/CO值 ,比较各试剂S/CO值的线性范围 (可报告范围 )和灵敏度。 结果 AXSYM、Murex和科华试剂检测HBsAg的线性范围分别为 0 .0 31～ 4 0 0 .0、0 .0 6 3～ 2 5 .0 0和 0 .5 0～ 5 0 .0 0ng/ml。在急性乙肝组和重症乙肝组中 ,乙肝e抗原 (HBeAg)阳性患者的HBsAgS/CO值显著高于HBeAg阴性患者 (P <0 .0 5 ) ,但在慢性乙肝、肝硬化和肝癌组中差异无显著性 (P >0 .0 5 )。结论 AXSYM测定HBsAgS/CO值的可报告范围较广 ,可直接向临床报告 ,并可作为动态观察急慢性乙肝患者病毒复制状况。

Objective To compare the sensitivity and working ranges of the S/CO ratio of HBsAg by different methods and reagents, and study the clinical significance of the S/CO ratio of HBsAg in patients with HBV infection. Methods Different standards of HBsAg were mensurated by AXSYM and ELISA, and the sensitivity and working ranges were compared depending on the results of the S/CO ratio. Results The working ranges of HBsAg of AXSYM, Murex and Kehua were 0.031-400.00, 0.063-25.00 and 0.50-50.00 ng/ml. In the groups of acute hepatitis B patients and serious hepatitis B patients, S/CO ratio of HBsAg in the HBeAg positive patients were higher than that of the HBeAg negative patients ( P <0.05, P<0.01 ). Conclusions The result suggested the S/CO ratio of HBsAg tested by AXSYM is useful in diagnosis and treatment in patients with HBV infection.