固体脂质纳米粒降低雷公藤内酯醇肝毒性的实验研究

Experimental research on triptolide solid lipid nanoparticle for decreasing liver toxicity of triptolide

目的 探讨固体脂质纳米粒降低雷公藤内酯醇 (TP)对肝脏的毒性作用。方法 用雷公藤内酯醇固体脂质纳米粒 (TP-SL N)小鼠 ig60 d后 ,测定小鼠血清谷丙转氨酶 (AL T)、谷草转氨酶 (AST)和肝组织中的超氧化物歧化酶 (SOD)、谷胱苷肽过氧化物酶 (GSH-Px)的活性以及丙二醛 (MDA)的含量。结果 与空白组相比 ,TP-SL N对 AL T,AST,SOD,GSH-Px,MDA的活性或含量差异无显著性 ;中、高剂量 (2 0 ,3 0μg/ kg)的 TP组则显著降低 SOD和 GSH-Px 的活性 ,并能显著提高 MDA,AL T和 AST的含量。结论 TP-SL N能降低 TP对肝脏的毒性。

Object To study the mechanism of triptolide solid lipid nanoparticle (TP-SLN) for decreasing liver toxicity of triptolide (TP). Methods With ig three doses of TP-SLN to mice for 60 d, the A LT, AST activities in serum and superoxide dismutase (SOD), glutathione peroxida se (GSH-Px) activites and malondialdehyde (MDA) contents in liver were determin ed. Results The activities of ALT, AST, SOD, GSH-Px and conten t of MDA between experimental group and blank group did not have remarkable diff e rence. However, the activites of ALT, AST, SOD, GSH-Px for the middle- (20 μg /kg) and high-dose (30 μg/kg) group were higher and the contents of MDA were lower than the experimental group. Conclusion TP-SLN can decrease the liver toxicity of TP.