对数期继代对南方红豆杉细胞培养动力学的影响

Effect of subculture at logarithmic phase on kinetics of cell cultures of Taxus chinensis var. mairei

目的 解决南方红豆杉细胞生长缓慢及代谢水平低下的问题。方法 对对数期 (2 0 d)和静止期 (3 0 d)的红豆杉细胞分别进行继代 ,在整个生长周期中测定了培养基中的碳源、氮源、磷酸盐的变化并分析了红豆杉细胞生长及紫杉醇的合成情况。结果 对数期继代细胞吸收碳源和硝态氮早于静止期继代的细胞 ,且前者的比生长速度是后者的 1.5倍 ,紫杉醇含量提高了近 4倍。

Object To solve the problem of low growth rate an d metabolism level in Taxus chinensis var. mairei (Lemée et Lévl.) Cheng et L. K. Fu Methods Cells on logar it hmic phase (20 d) and stationary phase (30 d) were subcultured individually. Th euptake of carbohydrate, nitrogen and phosphate from the medium was examined, th e growth rate of Taxus cell and the synthesis of taxol were anal y zed too during the whole growth period Results The Taxus cell subcultured at logarithmic phase uptook carbohydrate and nit rate earlier than that subcu ltured at stationary phase. The special growth rate of the former was 1.5 folds of that of the latter. The production rate of taxol was increased by nearly 4 ti m es Conclusion Subculturing at logarithmic phase will benefi t the biomass accumulation and the taxol synthesis