人视网膜色素上皮细胞的培养

Culturing of Fetal Retinal Pigment Epithelium

目的 :培养人视网膜色素上皮细胞 ,为采用视网膜色素上皮移植治疗色素变性奠定基础。方法 :用胰酶消化法获取人胎儿视网膜色素上皮细胞进行原代及传代培养。角蛋白抗体免疫细胞化学染色鉴定细胞。结果 :视网膜色素上皮细胞在体外生长良好 ,胞浆内含有丰富的色素颗粒。抗角蛋白反应阳性。结论

Objective: To establish retinal pigment epithelium (RPE) transplantation for further study on treating retinal pigmentosa, fetal RPE cells were obtained and cultured. Methods: The fetal RPE cells were cultured with trypsin enzyme digesting technique and passaged in vitro .The immunocytochemical staining with anti cytokeratin were performed for cell differentiation. Results: RPE cells grew well in vitro . Primary cultured RPE cells contained large amount of pigments.The cells were positive with anti cytokeratin staining. Conclusion: RPE cells cultured in vitro provids an ideal donor for cell transplantation. [