益骨胶囊对成骨细胞增殖影响的时效及量效关系研究

Time-effect and Dose-effect Relationships Study on the Effect of Benefiting-bone Capsule on Osteoblasts Proliferation

目的 :运用中药血清药理学方法 ,观察益骨胶囊含药血清对新生大鼠颅骨成骨细胞 (OB)体外增殖的影响 ,并探讨用于OB药效观察的益骨胶囊含药血清制备条件。方法 :雌性 12月龄SD大鼠 80只 ,随机分为生理盐水组、益骨胶囊低剂量 (11 6g/kg)、中剂量 (34 8g/kg)、高剂量 (10 4 4g/kg)组 ,每日灌胃一次 ,连续 12天 ,分别在灌胃后的 0 5h、1h、1 5h、2h采血 ,分离血清 ,用含不同浓度益骨胶囊的血清培养SD新生大鼠颅骨OB ,MTT法检测细胞增殖情况。结果 :末次灌胃后 1 5h采血组的吸收度 (A)值明显高于各组其它时间 (P <0 0 5 ) ;不同剂量各浓度的益骨胶囊含药血清对体外培养的OB增殖均有显著的促进作用 ,其中以高剂量组的稀释比为 2 5 %的血清浓度最佳。结论 :益骨胶囊含药血清对OB增殖具有明显的促进作用 ;益骨胶囊用于OB药效观察的大鼠含药血清制备条件以人等效剂量 9倍连续 12天灌胃、末次灌胃后 1 5h采血 ,血清添加浓度以 2 5 %为宜。

Objective: To investigate the effect of rats sera containing Benefiting-bone Capsule (BBC) on the cell proliferation of skull osteoblast in neonatal SD rats. Methods: Forty female SD rats, aged 12 months, were randomly divided into 4 groups including N. S. group, low dosage, middle dosage and high dosage BBC group, the sera were obtained at different times. MTT method was used to determine the effect of BBC on osteoblasts proliferation. Results: The MTT value reached its highest point 1.5 hours after oral administration (P<0.05). MTT of high dosage BBC met the maximum value with the concentration of 25% (P<0.05). Conclusions: The rats sera containing BBC can promote proliferation of osteoblast.