中国汉族、布依族和壮族群体中聚腺苷二磷酸核糖聚合酶-1基因多态性

Human PARP-1 Gene Polymorphisms in Chinese Han, Buyi and Zhuang Populations

【目的】探讨人类聚腺苷二磷酸核糖聚合酶-1(PARP-1)5个外显子核苷酸多态性。【方法】采用聚合酶链式反应-单链构象多态性(PCR-SSCP)和银染技术检测3个民族(汉族、布依族和壮族)634名正常人PARP-1基因多态性。【结果】在3个民族634名正常人血标本的5个外显子扩增产物SSCP电泳条带中,219名汉族人PARP-1基因5个外显子均未检出多态性条带;203名布依族和212名壮族人PARP-1基因的5个外显子扩增产物中分别有2例的外显子20检出1条多态性条带,其余4个外显子扩增产物未见多态性条带。【结论】PARP-1基因第20外显子上可能存在多态性。

Objective] To investigate nucleotide polymorphisms of five exons in human poly(ADP ribose)polymerase 1(PARP 1) gene. PCR SSCP silver staining was used to test the polymorphisms of PARP 1 gene in 634 chinese Han, Buyi and Zhuangzu people( 219, 203 and 212 respectively) . Polymorphisms of 5 exons of PARP 1 gene was not detected in 219 Han people and a polymorphisms of exon20 of PARP 1 gene was detected in 203 Buyi and 212 Zhuangzu people by PCR SSCP. Other exons were not detected. [ Conclusion] Polymorphisms may exist in exon 20 of PARP 1 gene.