血管内皮生长因子及其受体在舌癌细胞中表达及意义

Expressions of Vascular Endothelial Growth Factor and its Receptors in Human Tongue Carcinoma Lines

【目的】探讨人舌癌细胞株血管内皮生长因子(VEGF)及其受体KDR、Flt-1的表达,进一步认识VEGF在舌癌生长过程中的作用机制。【方法】以人脐静脉血管内皮细胞ECV304和小鼠成纤维细胞系L929作为对照,应用免疫组化染色和RT-PCR方法,检测体外培养的人舌癌细胞系Tca8113、GNM中VEGF及其受体的表达。【结果】GNM、Tca8113中皆有VEGF表达,Tca8113表达KDR和FLT-1,GNM表达KDR。【结论】在舌癌的生长过程中可能存在VEGF的自分泌机制。

Objective] To investigate the expressions of vascular endothelial growth factor(VEGF) and its receptors in human tongue carcinoma cell lines Tca8113 and GNM. Immunohisto chemical staining and reverse transcriptase polymerase chain reaction (RT PCR) were used to detect the mRNA and expressions of VEGF and its receptor :VEGFR1(Flt 1) and VEGFR2(KDR) in two human tongue carcinoma cell lines: Tca8113 and GNM, compared with that of ECV 304(human umbilical vein endothelial cells ) and L929 (mouse fibroblast). GNM? Tca8113 express VEGF protein . mRNA and proteins of KDR and Flt 1 could be detected in Tca8113, KDR mRNA and proteins could be detected in GNM. [ Conclusion] The expressions of FLT 1 and KDR suggest that VEGF may be an autocrine growth factor for human tongue carcinoma, at least for cell lines in vitro.