平喘合剂对实验性哮喘豚鼠嗜酸细胞阳离子蛋白、白细胞介素-5等的影响

Effect of Pingchuan Mixture on Eosinophil Cation Protein and Interleukin-5 in Experimental Guinea Pigs with Asthma

目的 :观察平喘合剂对哮喘豚鼠血浆嗜酸细胞阳离子蛋白 (ECP)、支气管肺泡灌洗液 (BALF)中白细胞介素 5 (IL 5 )水平及炎症细胞计数变化的影响。方法 :采用常规计数法测BALF中嗜酸粒细胞、中性粒细胞、淋巴细胞计数 ,运用ELISA法测IL 5 ,放免法测ECP。结果 :治疗后平喘合剂、地塞米松组嗜酸粒细胞、中性粒细胞、淋巴细胞数量 ,ECP、IL 5水平比治疗前明显降低 (P <0 0 5或P <0 0 1) ,平喘合剂、氨茶碱、地塞米松、定喘止咳片各组间比较 ,差异均无显著性。结论 :平喘合剂可降低炎症细胞计数、ECP、IL 5而治疗哮喘。

Objective:To observe the effect of Pingchuan Mixture (PCM) on plasma eosinophil cation protein (ECP), interleukin-5 in bronchial alveolar lavage fluid (BALF) and inflammatory cell count in experimental guinea pigs with asthma. Methods:The eosinophil, neutrophil, lymphocyte count were conducted by conventional method, IL-5 was detected by ELISA and ECP determined by RIA. Results:Levels of eosinophil, neutrophil, lymphocyte, ECP and IL-5 after treatment were significantly lower than those before treatment, the difference between groups treated respectively by PCM, aminophylline, dexamethasone and Dingchuan Zhike Tablet was insignificant. Conclusion:PCM could treat asthma by reducing the inflammatory cell count, ECP and IL-5.