人涎腺多形性腺瘤细胞体外培养及其生物学特性

Cell culture of salivary pleomorphic adenoma:Morphological and biochemical characterization

目的 建立人涎腺多形性腺瘤细胞系。方法 取自腮腺多形性腺瘤患者的原发肿瘤进行体外培养。对培养的细胞进行活细胞观察和组织学染色。观察细胞生长状况 ,绘制生长曲线。采用免疫细胞化学检测细胞内特异性抗原标记物对细胞进行鉴定 ,并对肿瘤的致瘤性进行裸鼠异种移植实验。结果 多形性腺瘤体外培养细胞呈多边形或梭形 ,有的细胞围成腺腔结构 ,胞浆内富含粘液 ,形成空泡。有的成片排列 ,细胞外有粘液样物质。细胞的生长曲线较平缓 ,无明显快速生长期。肿瘤性腺上皮细胞角蛋白阳性 ,肿瘤性肌上皮细胞肌动蛋白阳性。结论 建立的涎腺多形性腺瘤有限细胞系 (SPA - 0 2 ) ,保留了原代细胞的特点 。

Objective To establish the cell line of salivary pleomorphic adenoma(SPA).Methods The sample was obtained from a male patient with parotid pleomorphic adenoma.The morphologic features of SPA cells were obtained by using histological observation of live cells.Growth curve was made following the 5th passage cells.SPA cells were identified by means of immunohistochemistry.Then animal inoculation was performed to detect the characterization of SPA cells.Results SPA cells were spindle-shaped or polygonal in vitro,however,neoplastic adenoepithelial cells formed gland lumen with mucus in cytoplasm,while neoplastic myoepithelial cells were arranged in mass with rich mucoid substance in the stroma.The growth curve was smooth and showed no quick growth phase.The antibody against cytokeratin( 52.2KD ) was labelled in the cytoplasms of adenoepithelial cells,and myoepithelial cells were positive for the antibody against smooth muscle actin.Conclusion The finite cell line of salivary pleomorphic adenoma named SPA-02 had been established,and it maintained the properties of primary SPA cells.