高效液相色谱分析法检测培高力特血药浓度

High-performance Liquid Chromatographic Determination of Pergolide in Human Plasma

目的 :建立检测培高力特血药浓度的高效液相荧光色谱分析法。方法 :用氯仿抽提法进行样本预处理。采用WatersC18柱( 4 6mm× 2 5 0mm ,5 μm ) ,以乙腈 /氯化铵缓冲液 ( 96/4 )为流动相。样品荧光检测发射光波长 2 85nm ,吸收光波长 3 45nm ,内标为盐酸地尔硫。结果 :培高力特在 1～ 8ng·mL-1呈良好的线性关系。血浆样品回收率可达 70 %。结论 :本法灵敏、准确、重现性好。

Aim:To develop a method for the determination of pergolide in human plasma by high performance liquid chromatograph-fluorescence (HPLC-FLU).Methods:Diatiazem was selected an an internal standard.Serum sample was extracted with chloroform Water C 18 column (4.6 mm×250 mm,5 μm)was used.The mobile phase consisted of acetonitrile——0.01M ammonium chloride buffer,pH8.3(96:4).Effluents were monitored at 285 and 345nm.Results:The optimal working range was form 1 to 8 ng·mL -1 and the recovery of pergolide was over 70%.Conclusion:The method was proved to be sensitive,accurate and repeatable.