猪脾生长抑素阳性细胞和免疫细胞的发育

Ontogeny of somatostatin-positive cells and immunocytes in porcine spleen

用免疫组织化学技术研究猪脾生长发育过程中生长抑素 (SS)阳性细胞、IgA阳性细胞和酸性非特异性脂酶(ANAE)阳性细胞数量的变化。结果表明 ,出生时 ,猪脾SS阳性细胞数量较少 ,3日龄时数量显著增加 (P <0 0 5 ) ,2 0日龄则极显著增加 (P <0 0 1) ,并维持到 90日龄 ,12 0日龄时SS阳性细胞数量显著减少 (P <0 0 5 )。出生时 ,猪脾内就分布有IgA阳性细胞和ANAE阳性细胞 ,IgA阳性细胞数量于 2 0日龄呈显著上升趋势 ,4 5日龄达最高峰 ,于 90日龄极显著降低 (P <0 0 1) ;ANAE阳性细胞数量在 2 0日龄开始增加 ,4 5日龄时极显著增加 (P <0 0 1) ,12 0日龄时又显著降低 (P <0 0 5 )。以上结果表明 。

Immuno histochemical technique and histology method were used to investigate the changes of the number of somatostatin(SS) poistive cells,IgA positive and acid naphthyl acetate esterase(ANAE) positive cells in porcine spleen during growth.The results showed that the number of SS cells was few at birth. But it exhibited a significant elevation at 3 day old( P <0 05)and increased significantly( P <0 01)at 20 day old,then keeped high level to 90 day old.The number of SS positive cell decreased significantly( P <0 05)at 120 day old.Some IgA positive cells and ANAE positive cells were distributed in porcine spleen at birth and 3 day old.The number of IgA positive cells in porcine spleen exhibited a significant elevation tendency at 20 day old,reached the highest peak at 45 day old,then decreased significantly( P <0 01)at 90 day old.The number of ANAE positive cells in porcine spleen began to increase at 20 day old,increased significantly( P <0 01)at 45 day old and decreased significantly( P <0 05)at 120 day old.The results suggested that the ontogeny of SS cells play an important role in the development of immunocytes in porcine spleen.