摘要
本研究利用粳稻品种石狩白毛和籼稻品种明恢 6 3杂交的F2 分离群体共 116株 ,构建了含 88个共显性分子标记的连锁图谱 ,覆盖了水稻 (OryzasativaL .)基因组 140 6cM。分析了这些共显性标记在F2群体中的分离情况。结果表明 ,有 2 7个标记 (30 7%)的分离显著或极显著的偏离了预期的孟德尔比例(1:2 :1)而表现为异常分离 ,同时还发现了 6个异常分离的热点 ,即第 1染色体上RM 30 2 -RM 2 12 ,第 3染色体上RM 143-RM 85 ,第 6染色体上RM 5 4 0 -RM2 76 ,第 7染色体上PA1-A5 2 6 1和RM 4 32 -RM 4 5 5 ,第 12染色体上RM 5 19-RM 2 35。偏离分别指向两种亲本基因型。说明相关的异常分离因子以不同的方式控制雌雄配子的传递比例。
A rice genetic map was constructed which consists of 88 molecular markers covering 1406cM on the 12 linkage groups of rice by using an F 2 population from a cross between a japonica variety Shishoubaimao and an indica variety Minghui63. The segregation ratio of molecular markers was analyzed. Distorted segregation was found in 30.7% of the marker tested, which was indicated by significant deviation from the expected Mendelian segregation ratio (1:2:1) at 5% level. Six hotspot positions of distorted segregation were found on chromosome 1 (RM302-RM212), chromosome 3 (RM143-RM85), chromosome 6 (RM540-RM276), and chromosome 7 (PA1-A5261、RM432-RM455), and chromosome 12 ( RM519-RM235 ). The distorted markers showed the skewness favoring either the indica or the japonica parent. The results indicated that the male and female genotype transmitted to progeny with different frequency, which may be controlled by corresponding segregation distorting factors through different way.
出处
《分子植物育种》
CAS
CSCD
2003年第3期331-336,共6页
Molecular Plant Breeding
基金
广东省自然科学基金 (B2 0 1
2 0 0 0 30 2 3)项目资助
