动态监测SARS患者丙二醛一氧化氮和超氧化物歧化酶含量变化及其意义

Dynamic observation on changes of malondialdehyde,nitric oxide and superoxide dismutase in patients with severe acute respiratory syndrome and its significance

目的 :动态监测严重急性呼吸综合征 (SARS)患者丙二醛 (MDA)、一氧化氮 (NO)和超氧化物歧化酶(SOD)含量 ,并探讨其意义。方法 :采用硫代巴比妥酸比色法测定 MDA,硝酸还原酶法测定 NO,黄嘌呤氧化酶抑制法测定 SOD。分析早期、恢复期 SARS患者以及出院后 SARS随访者 ,一线未患 SARS的医务人员 ,健康体检者血清中 MDA、NO和 SOD含量的临床意义。结果 :SARS早期 MDA均值水平显著高于恢复期 (P<0 .0 0 1) ,恢复期均值均高于出院后随访时和健康人群 (P均 <0 .0 0 1)。 SARS早期 NO均值水平较高 ,恢复期和随访时次之。 SARS早期 SOD均值高于恢复期 (P<0 .0 0 1)、低于健康体检者 (P=0 .0 2 4 ) ,恢复期均值显著低于出院后随访时、一线未患 SARS医务人员和健康体检人群 (P均 <0 .0 0 1)。结论 :严重急性呼吸综合征的发病机制中病理损伤与自由基有关 ,SOD具有抗损伤作用。

Objective: To dynamically observe the changes of malondialdehyde (MDA), nitric oxide(NO) and superoxide dismutase(SOD) in patients with severe acute respiratory syndrome(SARS) and to explore its significance. Methods: MDA, NO and SOD were determined by using bathyran chromatometry, nitrate reductase method, xanthine oxidase inhibition method in SARS patients during the first, recovery and followup stage, the front health doctors and nurses and health people. The significance of their changes were analyzed . Results: The mean of MDA during the first stage was higher than that of recovery stage and the mean of recovery stage was higher than that of followup stage and health people (all P <0.001).The mean of NO during the first stage was higher than that of other stages. The NO means of recovery and followup stage were secondly higher. The mean of SOD during the first stage was higher than that of recovery stage and was lower than that of health people( P =0.024), the mean of SOD during the recovery stage was lower than that of followup stage, the front health doctors and nurses and health people (all P <0.001). Conclusion: The pathological injury in pathogenesis of SARS is related to free radicals. SOD has the capacity of antiinjury.