广州市交通警察血清p53蛋白的检测分析

Study on serum p53 protein in cops in Guangzhou city

目的 检测职业接触汽车废气的人群中血清p53蛋白的过表达情况 ,分析经常大量接触汽车废气是否增加了 p53基因异常的危险性。 方法 采用酶联免疫吸附试验检测血清中 p53蛋白的表达 ,采用卡方检验对工种与血清 p53蛋白表达之间的关系进行分析。 结果 交警外勤人员血清p53蛋白的过表达率为 5.74% ,高于内勤人员 ( 4 .89% ) ,但差异无显著性 (P >0 .0 5)。外勤人员工龄3 0年以上组血清p53蛋白过表达率为 12 .12 % ,高于 3 0年以下组 ( 5.3 6% ) ,P <0 .0 5,OR =2 .43 ,95%CI :1.11～ 5.3 3 ;平均每周接触汽车废气 40h以上组的血清 p53蛋白过表达率为6.89% ,远高于40h以下组的 4.16% ,二者的差异具有显著性 (P <0 .0 5,OR =1.71,95%CI :1.0 3～ 2 .81)。结论经常大量接触汽车废气可能增加了p53基因异常的危险性 。

Objective Serum p53 protein overexpressio n was detected in population exposed to traffic exhaust gas to study the relation between traffic exhaust gas and the increased risk in p53 gene mutution. Methods Serum p53 protein expression was measured by enzy me-linked immunosorbent assay. Relationship between different types of job and serum p53 protein overexpression were studied by pearson Chi-square tests. Results Results on serum p53 protein overexpression on j obs outside of office ( 5.74 %) were not signifincently higher than jobs insid e the office. However,it suggested that traffic police men ( 12.12 %) working outside of office,with whose length of service longer than 30 years had a sign ificant overexpression of serum p53 protein than the others ( 5.36 %) whose l ength of service was less than 30 years ( P< 0.05 , OR= 2.43, 95%CI: 1.11- 5.33 ). Overexpression rate of p53 protein appeared to be 6.89 % in the group whose average weekly exposure hours were more than 4 0 hours,which was significant higher than the group whose exposed hours were les s than 40 hours ( P< 0.05 , OR= 1.71 ,95%CI: 1.03- 2.81 ). Conclusion The result suggested that traffic exhaust gas was likely to cause mutation of p53 gene and increasing the incidence of lung ca ncer.