中国莱姆病螺旋体PD91重组OspC的鉴定和抗原性检测

Recombinant OspC identification and antigenicity detection from Borrelia burgd orferi PD91 in China

目的 重组中国莱姆病螺旋体PD91外膜蛋白C(OspC)并在大肠埃希菌中表达 ,用于早期莱姆病诊断的研究。方法 用聚合酶链反应 (PCR)扩增出PD91外膜蛋白C基因 ,定向克隆到表达载体PET 11D ,构建重组质粒PET 11D ospC。用PCR、限制性内切酶分析及序列测定等方法鉴定重组质粒。用WesternBlot检测其抗原性。结果 OspC基因被正确克隆到表达载体PET 11D中。序列测定结果证实与国外已报道的序列存在一定的差异。OspC具有强抗原性。

Objective To recombine OspC gene from Bo rrelia burgdorferi PD91 of China and expressed it in E.coli for early diag nosis of Lyme disease. Methods The OspC gene was amplified from the genome of Borrelia burgdorferi PD91 strain by polymorase chain reaction and reco mbined with plamid PET-11D. The recombinant plamid PET-11D-OspC was identifie d with PCR, restriction endonuclease analysis and sequencing. The antigenicity w as verified with Western Blot. Results OspC gene was cloned correctly into vector PET-1 1D. The resultant sequence was definitely different from the published sequence . The recombinant OspC seemed to have had strong antigenicity. Conclusion The findings laid basis for the studies on e arly diagnosis of Lyme disease.