人乳头状瘤病毒感染及端粒酶活性与宫颈癌的关系

Study on the correlation between the different papillomavirus type and telomerase in cervical cancer

目的 探讨不同型别人乳头状瘤病毒 (HPV )感染和端粒酶活化与宫颈癌的关系。方法 对 83例宫颈癌、47例宫颈上皮内瘤样病变 (CIN)及 10例正常宫颈应用TRAP PCR银染定性及TRAP PCR ELISA定量法检测端粒酶活性 ,PCR法检测HPV分型。结果 ( 1)端粒酶阳性率和HPV 16 18型阳性率 :宫颈癌 >宫颈CIN >正常宫颈 ,CINⅢ级 >CINⅠ、Ⅱ级 ;HPV 6 11型阳性率 :CINⅠ、Ⅱ级 >CINⅢ级。 ( 2 )宫颈癌中端粒酶阳性 :Ⅰ期 <Ⅱ期 <Ⅲ、Ⅳ期 ;中分化 >高分化 ;腺癌与鳞癌差异无显著性 ;HPV 16 18阳性 :Ⅰ期 <Ⅱ期 <Ⅲ、Ⅳ期 ;HPV 6 11阳性与临床分期、组织学分级及病理类型无关。 ( 3 )端粒酶阳性值 :宫颈癌 >CIN ,CINⅢ级 >CINⅠ、Ⅱ级 ;HPV 6 11拷贝数 :CIN >宫颈癌 ,CINⅠ、Ⅱ级 >CINⅢ级 ;HPV 16 18拷贝数 :宫颈癌 >CIN ,CINⅢ级 >CINⅠ、Ⅱ级。结论 HPV感染尤以 16

Objective To define a correlation between d ifferent human papillomavirus (HPV) types and telomerase activity in cervical c ancer. Methods Telomerase activity was detected by TRAP-PCR,and different HPV type was determined by PCR in 83 cervical cancer,47 cervical intr aepithelial neoplasia (CIN) and 10 normal cervix cases. Results With regard to positive rates of telomerase and HPV 16/18:the results were cervical cancer>CIN>normal cervix,CIN Ⅲ>CIN Ⅰ,Ⅱ ;with regard to HPV 6/11 positive rate:the results showed CIN Ⅰ,Ⅱ>CIN Ⅲ. Positive rates of telomerase cervical cancer and HPV were bearin g on grading and staging,but they did not correlate with histologic subtypes.Pos itive rate of HPV 6/11 had nothing to do with grading,staging and histologic pat terns.On expression strength of telomerase and HPV 16/18:the results showed cer vical cancer>CIN,CIN Ⅲ>CIN Ⅰ,Ⅱ. Regard to HPV 6/11'expression strengt h: the results showed CIN Ⅰ,Ⅱ>CIN Ⅲ, CIN>cervical carcinoma. Conclusion HPV 16/18 infection seemed to have played a n important role in carcinogenesis of cervical lesions by activation of telomera se.