血管内皮细胞对骨髓基质细胞促成骨作用的研究

Research in Use of Vascular Endothelial Cells to Promote Osteogenesis of Marrow Stromal Cells

在骨组织工程研究中 ,因营养血管长入材料非常缓慢而影响材料深部的成骨作用一直是困扰科研人员的难题之一。骨髓基质细胞能分泌血管内皮生长因子 (VEGF) ,内皮细胞可以产生骨形态发生蛋白 (BMP) ,因此我们设想将骨髓基质细胞和内皮细胞联合种植在生物材料上构建组织工程化骨 ,则可能在促进成骨的同时 ,又促进局部血管生成 ,满足成骨过程的营养需要。在本实验中我们比较了内皮细胞培养液作用下的骨髓基质细胞、经诱导的骨髓基质细胞和未处理的骨髓基质细胞之间碱性磷酸酶 (AL P)活性和骨钙素 (OCN)分泌量的差别 ,结果表明内皮细胞培养液作用下的骨髓基质细胞和经诱导的骨髓基质细胞之间碱性磷酸酶活性和骨钙素分泌量无统计学差异 ,但均明显高于未处理的骨髓基质细胞 (P<0 .0 1) ,说明内皮细胞培养液中的分泌因子对骨髓基质细胞具有促成骨作用 ,可以达到和成骨诱导液相同的效果。

Osteogenesis deep in the materials is postponed because the blood vessels grow into the materials very slowly. It is one of the key challenges to the bone tissue engineering researchers. However, it was found that marrow stromal cells can produce vascular endothelial growth factor (VEGF) while endothelial cells produce bone morphogenetic protein (BMP). So we presume if we have co-culture of marrow stromal cells and endothelial cells on the biomaterials, osteogenesis and angiogenesis may be promoted simultaneously. In this experiment, we compare the alkaline phosphatase (ALP) activity and osteocalcin (OCN) production of three groups:the marrow stromal cells under activation of endothelial cells culture fluid, the induced marrow stromal cells, and the untreated marrow stromal cells. The results reveal that the ALP activity and OCN production of the marrow stromal cells under activation of endothelial cell culture fluid and the ALP activity and OCN production of the induced marrow stromal cells do not show statistically significant difference, but they are significantly higher than those of the untreated marrow stromal cells (P<0.01). It is concluded that the VEGF in the endothelial cell culture fluid can promote osteogenesis as effective as osteogenic induction medium can do.