P27蛋白与调控因子cyclinE在结肠肿瘤组织中的表达

Expression of P27 Protein and Cyclin E in Colon Cancer

背景与目的:细胞调控与肿瘤发生、发展的关系是肿瘤研究的一个热点,肿瘤细胞调控因子也是肿瘤的重要预后因子。P27蛋白和cyclinE是细胞周期负性调控因子,到目前为止它们在肿瘤中所起的作用尚未十分清楚。本研究拟探讨P27蛋白与cyclinE在结肠肿瘤组织中的表达特征,以及与肿瘤特异性生长因子(tumorspecialgrowthfactor,TSGF)的关系。方法:正常结肠组织23例,结肠息肉28例(炎性息肉13例,腺瘤性息肉15例),结肠癌18例。上述病例常规病理检查确诊。用免疫组化方法检测所有标本中P27蛋白与cyclinE的表达以及与TSGF的关系。结果:P27蛋白和cyclinE在正常结肠组织、炎性息肉和腺瘤性结肠息肉组织中呈阳性表达,主要位于腺细胞的胞浆和细胞外基质。在结肠癌组织仅有少量表达,并且仅在少数腺样细胞的胞浆内。TSGF在结肠癌组织中的含量(117.30±57.02)明显高于正常组织(64.16±27.50)(P<0.01),但与炎性息肉组织(92.50±47.90)相比,差异无显著性(P>0.05)。结论:P27蛋白和cyclinE参与肿瘤的调控过程,P27蛋白和cyclinE表达下降提示结肠癌的可能。

BACKGROUND &OBJECTIVE:P27 protein and cyclin E were negative cell cycle regulators. Until the present, the influence of P27 protein and cyclin E on progression of colon cancer was unclear. The aim of this study was to observe the expression features of P27 protein and cyclin E in the tissues of colon neoplasms, and to investigate the relationship between colon neoplasms and tumor special growth factor (TSGF). METHODS: Sixty nine cases of samples included 23 normal tissues, 28 colon polyps (13 inflammatory polyps and 15 adenomatous polyps), and 18 colon carcinomas. The location and expression of P27 protein and cyclin E were determined using immunohistochemical method in all samples. These samples were diagnosed using formal pathological techniques simultaneously; the relationship between colon neoplasms and TSGF was also investigated. RESULTS: The positive signal of P27 and cyclin E was found mainly in the cytoplasm and extracellular matrix of normal colon tissues, inflammatory polyps, and adenomatous polyps. Less amount of postive expression product of P27 protein and cyclin E was observed in colon carcinoma cells; and the positive signal was only located in the cytoplasm of gland like cells. The content of TSGF in colon carcinoma tissues was significantly higher than that in normal tissues (117.3±57.02 versus 64.16±27.5,P< 0.01), but there was no significant difference between colon carcinoma tissues and inflammatory polyp tissues (117.3±57.02 versus 92.5±47.9,P >0.05). CONCLUSION: P27 protein and cyclin E participate in the adjustment process of colon neoplasm occurrence and progression. The reduced expression of P27 protein and cyclin E may indicate the possibility of colon carcinoma.