重金属污染土壤毒性的发光菌法与斜生栅藻法诊断

Toxicity Assessment of Soil Contaminated by Heavy Metals Using Algae Growth Inhibition Test and Photobacterim Phosphoreum Assay

应用斜生栅藻(Scenedesmus obliquus)和明亮发光杆菌T3(Phyotobacterium Phosphoreum)对重金属污染土壤的毒性进行诊断,结果表明,斜生栅藻的增长率和发光菌的相对发光度与土壤中的重金属含量明显相关,并且随重金属投加量的增加,其增长率和发光度逐渐降低,对3种测试参数进行比较,发现采用细胞数增长率作为土壤毒性的检测指标比采用光密度增长率和相对发光度更为敏感。复合污染情况下,由于金属间的协同作用,使土壤的毒性比单一重金属污染时有明显的增强,从而为污染土壤的优先修复提供了理论依据。

The toxicity of soil artificially contaminated by heavy metals was assessed with Scenedesmus obliquus and Photobacterium Phosphareum . The results showed that the growth rate and the luminescence were positively related to the concentration of heavy metals added to the soil, and they were decreased with the increasing concentration of heavy metals. Three parameters were chosen and the sensitivities were compared. It was found that the cell growth rate was more sensitive than OD growth rate and relative luminescence. This study also indicated that under the complex pollution, the toxicity of heavy metals in soil was stronger than that in the case of soil contaminated by single metal.