大鼠肝缺血预处理对肝缺血再灌注所致肝外脏器损伤的保护作用

Protective effect of liver ischemic preconditioning on the extrahepatic organs injury induced by liver ischemia/repurfusion in rats

目的 探讨大鼠肝缺血预处理 (IP )对缺血再灌注 (I/R )致肝外主要脏器损伤的保护作用。方法 72只SD大鼠随机分为IP ,I/R组及S(假手术 )组 ,每组 2 4只。建立IP及I/R模型后观察术后 2 ,2 4h及 1周时小肠、胰腺、心肌、肾、肺、脑及骨骼肌组织病理学改变。结论 (1)小肠组织损伤程度 :在 2h及 2 4h时IP组及I/R组显著高S组 (P <0 .0 1) ,且I/R组显著高于IP组 (P <0 .0 1)。 (2 )肾脏组织损伤程度 :I/R组在 2 ,2 4h及 1周时均显著高于S组 (P <0 .0 5 ,P <0 .0 1,P <0 .0 5 ) ,IP组 2 4h及 1周时显著低于I/R组 (P <0 .0 1,P <0 .0 5 )。 (3 )IP组胰腺和肺组织损伤虽较I/R组有所以改善 ,但无统计学差异 (P >0 .0 5 ) ,但IP组及I/R组均显著高于S组。 3组的脑、心肌及骨骼肌组织未见明显损伤。结果 大鼠肝脏缺血预处理能有效减轻肝缺血再灌注对小肠及肾脏的损伤。

Objective To study the protective effect of liver ischemic preconditioning on the extrahepatic organs injury induced by liver ischemia/repurfusion in rats. Methods Seventy-two Sprague-Dawley rats were randomly assigned into group IP,group I/R and group S (sham-operation group), each group had 24 rats. After ischemic preconditioning and ischemia/repurfusion animal models were set up,the pathological changes of small intestine, pancreas, myocardium, kidney, lung, brain and skeletal muscle tissues were observed at 2h,24h and 1week,respealively. Results (1) The degree(s) of small intestinal injury: at 2h and 24h, The injury in group IP and group I/R were significantly higher than that in group S (all P <0.01); and at 2h, it in group I/R was significantly higher than that in group IP ( P <0.05). (2) The degree of renal injury: in group I/R, this was significantly higher than that in group S at 2h, 24h and 1 week (P <0.05, P <0.01 and P <0.05, respectively); and at 24h and 1 week, in group IP it was significantly lower than that in group I/R ( P <0.01 and P <0.05, respectively). (3) Although the injury of the pancreas and lung in group IP were lighter than those in group I/R, no statistical differences showed between the 2 groups ( P <0.05);but in group I/R and IP group were significantly severe than those in group S( P <0.05). And no obvious injuries were observed on the brain, myocardium and skeletal muscle tissues in all the 3 groups. Conclusions Liver ischemic preconditioning can effectively ameliorate the injuries of the small intestine and kidney induced by liver ischemia/reperfusion in rats.