野山茶的短期毒性研究

Study on short-term toxicity of wild camellia

目的 观察野山茶对动物体重、血常规、血液生化指标及病理组织学的影响及食用安全性 ,为山茶饮品产业开发提供基础资料 方法 取大鼠 80只 ,雌雄各半 ,设 2 5 0 0、12 5 0、62 5mg kg·bw 3个剂量组和 1个空白对照组 ,每组 2 0只。采用灌胃法给予受试物 (每 10 0g体重 1ml) ,空白对照组给予蒸馏水 ,每周称体重 1次 ,并根据体重调整给受试物量 ,自由进食和饮水 ,连续 3 0d。第 3 1d处死动物 ,进行血象、血清生化指标检测以及肝、肾、胃肠等脏器病理组织学检查。 结果 高中剂量组的ALT和各剂量组的AST均较空白对照组明显升高 ;以及高剂量组的 3 0 %肝组织局部肝小叶中央带细胞轻度浊肿和 10 %大部分肝细胞弥漫性浊肿并见泡沫样变。

Objective To observe the impact of wild Camellia on the body weight,blood routine,biochemical indexes,pathology and histology of rat and obtain the asfety data and set the foundation for the sound development of wild Camellia industry Methods Eighty rats consisted of 40 male and 40 female rats were divided into 4 groups each comprised of 20 rats,i e three dose groups tested with 2500?1250 and 625 mg/kg body weight,respectively and a bland control group Wild Camellia was given to each rats in experimental groups(1 ml/100mg body weight) and distilled water was given to rats in control group for a succession of 30 days On d31 the animal were killed and examined Results The ALT in high dose group and the AST in other dose groups were obviously increased as compared with that of the control and mild swelling in the 30% liver lobult cells of local liver tissue in high dose group were noticed While diffuse swelling was observed in 10% liver cells with foamy changes Conclusion Wild Camellia is toxic to the liver of rats over an experimental period of 30 days