手性交联毛细管柱的研制

Study on Crosslinked Chiral Capillary Columns

自从Gil-Av等人首次成功地用气相色谱法分离对映体以来,手性固定相的发展非常迅速,国外已出现了多种性能优良的手性固定相。我们从1984年开始从事“用气相色谱法分离对映体”的研究,已研制出几种性能优良的二酰胺类手性固定相。 交联毛细管柱具有耐温性好和耐溶剂冲洗的特点,并且能用于超临界流体色谱中,因此,手性交联毛细管柱的研制引起了人们的极大兴趣。 手性固定相不同于一般固定相,若交联条件不当会引起固定相的外消旋化而失去拆分对映体的能力。因此。

Chiral stationary phase OV-225-L-Val-tert-butylamide has been crosslinked on both glass and fused silica capillaries. The features of the columns in terms of enantioselectivity, theoretical plates per meter, thermal stability and acid/base ratio were investigated. The columns have been used for the separation of amino acid enantiomers in supercritical fluid ehromatography for. several months without detectable bleeding.