高效液相色谱双波长检测测定复方氯丙嗪片中有效成分的含量

Determination of the Active Ingredients in Tablet Chlorpromazine Hydrochloride Composite by High Performance Liquid Chromatography with Dual-Wave- length Detection

本文利用Waters490型检测器的特性,将双波长分光光度法原理用于严重重叠的色谱峰的检测,分别测定了复方氯丙嗪片中盐酸氯丙嗪和盐酸异丙嗪的含量。工作曲线线性良好,与文献报道方法比较,结果相符。 基本原理 Waters 490型检测器有4个检测通道(Channel),每一通道为一检测单元。将检测波长λ_1、λ_2分设在通道1、2,两通道的10mV输出(+)端分别连接于740数据处理机的(+)、(-)端。这样记录的信号为两通道的检测信号之差。

On a Waters 490 UV-detector, the principle of dual-wavelength spectrometry was applied to detect unresolved peaks in HPLC. The amounts of chlorpromazine hy-drochloride ( Ⅰ ) and promethazine hydrochloride ( Ⅱ ) in tablet chlorpromazine ingredient were determined and satisfactory results were obtained. Comparison between the results from this method and those from literature is illustrated.