反相离子对色谱用于尿液中假尿苷和肌酐的测定

Simultaneous Determination of P(?)dine and Creatinine in Urine by Ion-Pair Reversed-Phase High Performance Liquid Chromatography ( RP-HPLC )

假尿苷(PU)可作为肿瘤的生物学标志,并可据其变化监测疗效。以往测定血及尿液中PU需经苯硼酸亲合色谱预处理样品,操作繁琐费时。另外,对分析结果必须求出PU与肌酐的比值才有意义,且需两种不同方法检测两种物质的含量,所得结果的精确性较差。本文提出,标本不经亲合色谱预处理。

In the present paper, a rapid, accurate and simple method for simultaneous determination of pseudouridine (PU) and creatinine in urine by ion-pair RP-HPLC without the need of prstreatment of urine with boronate affinity gel is proposed. PU and creatinine are separated by using 0. 05mol/L phosphate buffer, 5rmnol/L octane-snlphonic acid, 3%methanol as the mobile phase at pH 5. 8 and detected at UV 250nm. The linear relationships between the peak areas and the quantities of PU and oreatinine are good. The correlation coefficients of the calibration curves for PU and creatinine are 0. 996 and 0. 947,respectively.