自动变换波长反相高效液相色谱法同时定量测定生物样品中磷酸肌酸和腺嘌呤核苷酸

Simultaneous Determination of Creatine Phosphate and Adenine Nucleotides in Muscles by Reversed-Phase High Performance Liquid Chromatography (HPLC ) with Automatic Adjustment of Wavelength

腺嘌呤核苷酸(ATP、ADP和AMP)及磷酸肌酸(CP)是生物能量转换的主要成分。测定这些成分将 有助于了解组织器官的功能状态及某些能提高缺氧耐力的药物与改善能量代谢的可能关系。HPLC可以同时测定多种核苷酸成分。

A method for the determination of creatine phosphate(CP)and adenine nucleotides (ATP, ADP and AMP) in the muscles of mice by reversed-phase HPLC is presented. The separation was achieved at room temperature with a Cu column,one step gradient elution and an automatic adjustment of wavelength. The solvent A included 30mmol/L KH2PO4 4mmol/L PICA.pH 6. 0 and 5 % (V/V) methanol. The solvent B included 30mmol/L KH2PO4,4mmol/L PICA,pH 6. 0 and 19% (V/V) methanol. This method has the advantages of simple sample preparation,low cost,less interference and high sensitivity. The results obtained were consistent with those from other methods.