吲哚衍生物的薄层色谱分离方法

A Study on the Thin-Layer Chromatography(TLC) Separation of Indole Derivatives

N-烷基化取代吲哚是吲哚苯酞类压敏染料的重要中间体,通过非N-烷基化取代吲哚进行相转移催化合成。为了采用薄层色谱法跟踪相转移催化反应,必须寻找取代吲哚在N-烷基化前后两者薄层色谱分离的条件。文献较多地报道了吲哚母体及含羧基、羟基、氨基、氰基、酯基等极性吲哚衍生物在有关展开剂中的比移值R_f,而对极性相差很小的N-烷基化和非N-烷基化取代吲哚的分离条件,尚未见详细报道。

The conditions for TLC separation of N-alkylated and non-N-alkylated indotes have been investigated in this work. Their R, values in the simple solvent and mixed solvent conditions were determined, respectively. The successful developing agents and the detection limits of these indoles with some detecting agents have also been found.