人血浆中反式维甲酸的高效液相色谱法测定及其药代动力学的研究

Determination of Trans-Retinoic Acid (TRA)in Plasma with High Performance Liquid Chromatographic (HPLC)Method and the Study of Its Pharmacokinetics

反式维甲酸(all-trans-Retinoic Acid,简称TRA)是Vit A的羧化衍生物,1980年首次证实对白血病细胞具有诱导分化作用,被认为是治疗白血病的一种新的有效药物。 我院从1986年开始将TRA应用于临床,为了指导临床更合理地应用TRA,采用高效液相色谱法,首先选择家兔为动物试验模型,作为药代动力学研究的预试验,为成功地研究人血浆中TRA药代动力学提供了可靠的实验方法和条件。

All-trans-Retinoic Acid (TRA ) is a analog of vitamin A effective in reversing preneoplastic lesions in both humans and animals. To study its pharmacokinetics, we developed a rapid ,sensitive.and precise HPLC method. The analysis conditions were Nuoleosil ODS column, 7μm, 200 × 4. 6mm i. d. , mobile phase acetonitrile : amino acetate (75 : 25 ), detector UV at 340nm. Average recovery was 97. 6 ± 5. 8%. The detection limit for TRA was 6. 66 × 10-2 μmol/L.