四个春箭舌豌豆品系原种及第三代过氧化物酶同工酶分析

POD isozyme analysis for F_0 and F_3 of four Vicia sativa lines

应用聚丙烯酰胺凝胶电泳技术,对引自国际干旱农业研究中心的4个春箭舌豌豆品系原种(F0)和在甘肃省甘南藏族自治州经过3个世代选育的F3代,进行幼苗下胚轴过氧化物酶同工酶分析。结果表明,2505品系F3代在相对迁移率(Rf)为0.725位置和原种相比酶带缺失,而Rf值为其它位置处谱带颜色均较原种为浅;2556F3代在Rf值为0.725处呈现酶带,而原种该谱带缺失,其它位置各谱带明显较原种为深;2560F3代在Rf值为0.211和0.266位置处酶带变宽,颜色加深;2566F3代在Rf值为0.725处呈现酶带而原种该谱带缺失,在Rf值为0.211和0.266处2酶带较原种位置偏上,颜色偏深。供试8个品系过氧化物酶同工酶酶谱与对照品种333/A相比,均存在较大差异。由此对以上4品系原种和F3代呈现出的宏观上的生态适应性提供了分子水平上遗传变异依据。

Four lines of Vicia sativa were introduced from the International Center for Agricultural Research in Dry Areas (ICARDA).The codes of the introduced lines were 2505, 2556, 2560 and 2566. They were evaluated and selected for adaptation to local environments, and high seed and herbage yields, under alpine grassland conditions at Xiahe County in the Gansu province of China. Performance of F3was significantly better than its parent generation. Therefore, seedling hypocotyl POD isozyme PAGE was used to study the genetic mechanism of F0 and F3of the 4 lines tested .The results showed that the mapping of POD isozyme PAGE was markedly different between F0and F3. For the line 2505, F3missed one band at the loci Rf 0.725 when compared to F0, and in other locations the bands of F3were lighter than those of F0. For the line 2556, F3presented one band at the loci of Rf 0.725 whereas this was missing for F0, and at other locations, the bands of F3were darker than those of F0. For the line 2560, the two bands at the loci Rf 0.211 and 0.266 of F3became wider and darker than those of F0. For the line 2566, F3presented one band at the loci Rf 0.725 which was missing in F0, and at other locations the bands of F3were darker than those of F0. 333/A (CK) POD isozyme mapping showed marked differences to tested lines. The study suggests that POD isozyme PAGE offers evidence of the ecological adaptability of F3of 4 lines of V.sativa at the molecular level. 