转基因抗虫保铃棉(Bollgard~ひ)检测方法研究

Detection Mothod of Exogenous Gene from Transgenic Bollgard~ひ Cotton

运用常规 PCR方法和实时荧光 PCR方法对转基因抗虫保铃棉外源基因 Ca MV 35 S启动子、NOS终止子、标记基因NPT II和目的基因 Cry IA(c)进行了检测。建立了快速、准确的抗虫保铃棉籽转基因成分的 PCR检测方法。

The exogenous CaMV 35S promoter, NOS terminator, NPT II and CryIA(c) gene in genetically modified Bollgard  cotton were detected by conventional PCR and real time PCR, so the accurate and fast PCR detection methods were conducted.