摘要
通过对鸡静脉注射卡介苗 (BCG)和脂多糖 (L PS) ,应用 L92 9细胞检测鸡体外周血清、脾细胞和外周血白细胞肿瘤坏死因子 (TNF)活性 ,表明外周血清 TNF活性最高 ,其次是脾细胞 ,而外周血白细胞 TNF活性最低。本研究也表明在正常鸡的外周血液中有少量的
After inoculating chicken with Bacille Calmette Guerin(BCG) and Lipopolysaccharide (LPS),the serum and the leukocytes of blood were separated, and splenic cells were collected. The tumor necrosis Factor (TNF) was detected with L929 cells. The results showed that the TNF had been induced in chickens by BCG and LPS, and the activities of TNF derived from the serum of chickens were the highest, and then those of TNF from splenic cells, and those of TNF from leckocyte were the lowest. A few TNF also were detected in the control chickens.
出处
《动物医学进展》
CSCD
2003年第5期84-86,共3页
Progress In Veterinary Medicine
基金
国家自然科学基金资助项目 (3976 0 0 6 1)
关键词
鸡
肿瘤坏死因子
活性
卡介苗
脂多糖
TNF
chicken
tumor necrosis factor(TNF)
induction
activity detection
