鸭瘟病毒的PCR检测及其产物分析

Ditection of Duck plague virus Using Polymerase Chain Reaction and Analysis of its Production

根据基因库中已有的鸭瘟 Ul6的序列 ,合成一对引物 ,对 3株鸭瘟病毒进行 PCR扩增 ,均扩增出大小约 42 0 bp的特异性片段。进行测序 ,结果表明 3个毒株扩增后的片段均为 42 1bp。经重复实验后确定最佳反应条件 ,按该条件对两例临床病料进行检测 ,结果均出现大小约为 42 0 bp的特异片段。PCR产物与 L ake Andes株的相关序列比较 ,广东毒株与 L ake

A pair of primer was designed and synthesized according to the sequence of Duck plague virus Ul6 gene.Using the primers,a 421 bp long DNA fragment of DPV was amplified.DNA sequencing showed that the PCR production of three strains was 421 bp long.The optimal condition was confirmed by the repetition of PCR.The PCR assay was application of two clinic samples,and the specific fragment of 421 bp long was also amplified.Alignment of the PCR fragment showed high identity with Lake Andes strain.