两种小麦的原生质体培养再生植株

PLANT REGENERATION FROM CELL SUSPENSIONDERIVED PROTOPLASTS OF WHEAT(TRITICUM AESLIVUM L.)

报道两种基因型小麦昌乐5号和山农587胚性悬浮细胞系的建立和原生质体再生植株。小麦昌乐5号和山农587的胚性愈伤组织继代一年以后形成部分颗粒状和粉粒状结构,可用于悬浮培养。当分散好、生长快的胚性悬浮细胞系形成后,即可作为原生质体培养的材料。较长时间的悬浮培养容易使材料的胚性丧失,昌乐5号的胚性悬浮细胞系20天左右建成,其原生质体再生植株的频率为14/10~5(再生植株数/植板的原生质体数);而山农587的悬浮培养物需5个月以后才能用于原生质体培养,其原生质体再生植株的频率仅为4/10~6。

Plant regeneration from embryogenic cell suspensions of wheat (cv. Chang-Le No.5 and Shannong 587)was described in this paper. The embryogenic cell suspension initiated from embryogenic calli which were induced from young embryos and subcultured for one year. The calli of var. Chang-Le were small granular in morphology and that of Shannong 587 were granular. The establishment of a fine dispersed and fast growth suspension for Chang-Le needed only 20 days, but for Shannong about five months. A short time in cell suspension subculture is beneficial to the maintenances of plant regeneration potential. The time for plant regeneration from protoplasts of Chang-Le was about three months and the frequency of differentiation into plant was 14/10~5(number of regenerated plants /number of protoplasts plated), but Shannong 587 needed six months for plant regeneration and had lower regeneration frequency(4/10~6).