液相色谱/质谱法分离鉴定碘化酪蛋白水解产物中的碘化氨基酸

Separation and Identification of Iodo-Amino Acids in Hydrolysate of Iodinated Casein by Liquid Chromatography/Mass Spectrometry

用WatersSymmetryShieldTM RP18柱 ,乙腈 (B ,含 0 .1%甲酸 )和水 (A ,含 0 .1%甲酸 )为流动相 ,0 .8mL min ,线性洗脱 (B从 10 %到 5 0 %) 4 0min ,液质联用 (LC MS) ,电喷雾电离源 (ESI+,SIR方式 ) ,对碘化酪蛋白水解物中的一碘酪氨酸 (MIT)、二碘酪氨酸 (DIT)、二碘甲腺原氨酸 (T2 )、三碘甲腺原氨酸 (T3 ,rT3 )和四碘甲腺原氨酸 (T4)进行了分离确证。同时测定了T3 、rT3 和T4的含量。

Using liquid chromatography/mass spectrometry (ESI+, SIR mode), phase B(acetonitrile, 0.1% formic acid), phase A(water, 0.1% formic acid) 0.8 mL/min of flow rate, a linear gradient from 10% to 50% of phase B and eluate for 40 minutes on a Waters Symmetry shield(TM) RP18 column, the 3-monoiodo-tyrsine(MIT), 3,5-diiodo-tyrsine(DIT), 3,5-triiodo-thyronine(T-2), 3,3'5-triiodo-thyronine(T-3), 3,3',5'-triiodo-thyronine(rT(3)) and 3,3',5,5'-tetraiodo-thyronine (T-4) in the hydrolytes of iodinated casein were separated and identified. The quantitations of T-3, rT(3) and T-4 were performed, simultaneously.