原生质体诱变选育透明质酸菌株及发酵条件的优化

Studies on the Protoplast Mutation of Hyaluronic Acid Producing Strainand the Optimization of Fermentation Condition

采用N 甲基 N′ 硝基 N 亚硝基胍(NTG)诱变原生质体,选育了一株产透明质酸的兽疫链球菌(Streptococcuszooepidemicus)H 35.并经过发酵条件的优化,确定了溶菌酶作用的最佳条件是0.6mg/mL溶菌酶高渗溶液,30℃水浴下酶解20min;H 35的最佳发酵条件是葡萄糖4.0%、酵母膏2.0%、NaCl0.2%、KH2PO40.2%、MgSO4·7H2O0.06%、初始pH7.0、35℃,200r/min、培养18h.发酵液中透明质酸的含量从最初的0.47g/L提高到优化后的1.43g/L.

The protoplast of  Streptococcus zooepidemicus was prepared and muted by NTG and strain H35 with high yield was obtained.The conditions of fermentation were optimized .The optimum concentration of lysozyme,temperature and time were 0.6mg/mL,30℃ and 20minutes respectively. The optimum culture conditions of producing HA: initial concentration of glucose 4.0%,yeast extract 2.0%,NaCl 0.2%,KH2PO40.2%,MgSO4·7H 2O 0.06%,the temperature 35℃,initial pH7.0,fermentation period 18hours.The highest yield of Hyaluronic acid increased from 0.47g/L originally to 1.43g/L.