摘要
对胀果甘草(GlaycyrrhizainflatBat)的愈伤组织培养及毛状根农杆菌介导的遗传转化进行了研究,并对培养物进行了甘草有效成分分析。结果表明,培养的愈伤组织及毛状根的薄层层析图谱与甘草药材的非常接近,其化学成分有相当的相似;在愈伤组织及毛状根中均含有甘草的主要有效成分甘草酸和甘草次酸;不同来源的愈伤组织中甘草酸的含量不同,其中以根来源的愈伤组织中甘草酸含量最高;愈伤组织中各种氨基酸的含量均高于甘草药材。
In this experiment, the author studied the callus tissue culture of the Glycyrrhiza inflat, and induced the hairy root of it by Agrobacterium rhizogenes R1601.Analysis of the callus,hairy root and crude drug powder of G. inflat indicated that they all contained the glycyrrhizic acid, glycyrrhetinic acid and various kinds of amino acid, and the amino acid contents in callus were more than those in the crude drug powder. The different callus had different abilities of producing glycyrrhizic acid, and the callus induced from the root was more suitable for synthesis of the glycyrrhizic acid.
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2003年第4期34-37,共4页
Journal of Hebei Agricultural University
基金
河北省教育厅2002年自然科学研究指导计划(Z2002112)
