影响马铃薯试管苗生长和基因转化因素的研究

STUDY ON FACTORS EFFECTING TEST TUBE SEEDLLING GROWTH AND GENE TRANSFORMATION ON POTATO

在马铃薯试管苗培养时 ,强光照 ( 6 0 0 0Lx)能促进试管苗的生长 ;多效唑抑制茎叶的生长 ,但可促进根系生长 ;较低苗龄 ( 3～ 4周 )的外植体可获得较高出芽率 ,外植体预培养

Factors affecting test tube seedling growth and gene transf ormation on potato are studied. It is found that the intensity of light (6000 Lx ) can promote growth, the multiple effect triazole (MET) inhibit the growth of stem and leave s but it can promote growth of the roots, and the younger seedling of explants h ave higher rate of germination, the explant of preculture 2 days can raise transfor mational rate.