摘要
为了更好地掌握代谢规律和代谢方式,代谢流作为一个关键而重要的变量需要测定.然而,由于代谢流测定的困难,在许多代谢研究中代谢流并没有被完全应用.在肌苷高产菌枯草芽孢杆菌中,利用得到的实验数据包括葡萄糖的消耗速率和代谢主产物以及副产物的形成速率,利用代谢通量平衡模型,得到糖酵解、三羟酸循环及磷酸戊糖途径的代谢流,并对其进行了分析.
It is well recognized that metabolic fluxes are the key variables that must be determined in order to understand metabolic regulation and patterns. However, owing to difficulties in measuring the flux values, evaluation of metabolic fluxes has not been an integral part of the most metabolic studies. Flux values for metabolites of glycolysis (EMP), tricarboxylic acid (TCA) cycle, and hexose monophosphate (HMP) pathway was obtained for batch cultures of inosine overproducing Bacilus subtilis by combining the information from the stoichiometry of key biosynthetic reactions with the experimental data on uptake rate of glucose and formation rates of metabolic product and byproducts.
出处
《南开大学学报（自然科学版）》
CAS
CSCD
北大核心
2003年第3期116-122,共7页
Acta Scientiarum Naturalium Universitatis Nankaiensis
关键词
肌苷
代谢流
计量系数
枯草芽孢杆菌
inosine
metabolic fluxes
stoichiometry
Bacillus subtilis
