二苯乙烯苷对胆碱能损伤拟老年性痴呆大鼠学习记忆功能和兴奋性的影响

Effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside, on learning and memory function and excitability in Alzheimer's disease rat induced by model cholinergic damage

目的研究何首乌有效成分二苯乙烯苷 ( 2 ,3 ,5 ,4’ 四羟基 二苯乙烯 β D 葡萄糖苷 ,TSG)改善胆碱能损伤拟老年性痴呆大鼠学习记忆功能的作用及对中枢兴奋性的影响。方法采用鹅膏蕈氨酸基底前脑注射建立拟老年性痴呆大鼠模型 ,实验动物分为假手术、模型、阳性对照药盐酸多奈哌齐 ( 1 2mg/kg)、TSG小剂量 ( 0 0 3 g/kg)、中剂量 ( 0 0 6g/kg)和大剂量 ( 0 12 /kg) 6组 ,灌胃给药 1个月后 ,分别用Morris水迷宫、通道式水迷宫、避暗反应及旷场分析对动物的学习记忆功能及兴奋性进行评价。结果TSG小、中剂量组均能显著缩短Morris水迷宫游出时间 (P <0 .0 5 ) ;TSG3个剂量组均减少通道式水迷宫的错误次数 (P <0 .0 1)。避暗反应显示 ,TSG各剂量组的成绩均优于模型组。旷场分析发现该药对模型大鼠兴奋性无显著影响。结论TSG具有改善胆碱能损伤拟老年性痴呆大鼠学习记忆功能 ,小、中剂量TSG对动物中枢兴奋性有一定的促进作用 ,而大剂量可能对动物中枢兴奋性有一定抑制作用。

ObjectiveTo investigate effects of 2,3,5,4'-tetrahydroxystilbene-2-O-β-D-glucoside (TSG), on learning and memory function and excitability in Alzheimer's disease rat model induced by cholinergic damage.MethodsBasal forebrain of rats were injured by ibotenic acid injection of 10μg each side. Rats were divided into six groups as the operation, model, positive control drug donepezil hydrochloride, TSG low (0.03g/kg), middle (0.06g/kg) and high (0.12g/kg) dose groups. These drugs were intragastrically administrated for 1 month The learning and memory function were determined with Morris water maze, channel water maze and step through tests, and the excitability was evaluated by open field analysis ResultsTSG (low, middle and high dose)significantly decreased the swimming time and error times ( P<0 05) in water maze test and TSG high dose improved the ability of passive avoidance response at step through test Open field test showed that there was no significant difference on excitability between each group ConclusionTSG can improve learning and memory abilities of Alzheimer's disease rat induced by cholinergic damage, TSG(low and middle dose)improve excitability of central nervous system in Alzheimer's disease rat model,but TSG dose maybe inhibite excitability of central nervous system.