苦参药材提取方法的LC/MS研究

Study on extraction of Sophora flavescens Ait. by HPLC-MS

目的 :苦参药材不同提取方法的化学成分分析。方法 :采用高效液相色谱 /电喷雾离子化质谱联用技术对苦参药材不同的提取方法进行研究。结果 :氯仿 浓氨溶液提取法 ,火溶液提取法 ,水 甲醇溶液提取法获取生物碱和黄酮依次为9,8和 12种。结论 :首次采用LC/ESI/MS联用技术对苦参药材的提取方法进行了研究 ,为中药苦参指纹图谱的建立提供了依据。

Objective:Chemical constituents of Sophora flavescems Ait. were studied by different extraction. Methods: Online HPLC/ESI/MS was used to study the different extraction. Results: numbers of alkaloids and flavone from Sophora flavescens Ait. were 9,8 and 12, respectively by means of chloroform strong aqua , water , and water methanol extraction. Conclusions: For the first time, we studied extraction of Sophora flavescems Ait by HPLC MS. This provided basic for study on fingerprints of Sophora flavescems Ait..