与云南烟草丛顶病害病原相关的病毒粒体性质的初步研究

Study of Virus Particle Associated With Pathogen of Tobacco Bushy Top Disease in Yunnan

目的 对与云南烟草丛顶病害病原相关的病毒粒体进行初步定性。方法 应用电子显微镜、PAGE -SDS电泳和甲醛变性电泳。结果 发现该病毒为一直径在 2 6nm左右的球状粒体 ,病毒外壳由 1种分子量在 5 5kDa左右的蛋白组成 ,病毒壳内核酸由 3种RNA组成。

A virus particle associated with tobacco bushy top disease inYunnan was analyzed by electromicroscopy and electrophoresis. An isometric virus particle with a diameter of 26 nm was obtained from the Aphid on infected tobacco plants. A coat protein band with a molecular weight about 55 kDa was obtained by PAGE-SDS electrophoresis, and three RNA bands were isolated from the virus particle.