摘要
目的 :探讨大鼠精索静脉曲张对睾丸生精细胞凋亡的影响 ,阐明精索静脉曲张引起不育的病理机制。 方法 :选用雄性SD大鼠 32只 ,随机分为假手术组、4 5d模型组、6 0d模型组及 90d模型组。采用肾静脉缩窄法建立精索静脉曲张动物模型 ,流式细胞术分别检测各组大鼠睾丸凋亡细胞数及各级生精细胞数。 结果 :假手术组未出现明显的凋亡峰 ,各模型组均出现明显的凋亡峰 ,且随着造模时间的延长凋亡峰增高。 结论 :精索静脉曲张可引起睾丸生精细胞大量凋亡 ,各级生精细胞数减少 。
Objective: To explore the effects of experimental varicocele on the apoptosis of spermatogenic cells in rats. Methods: Thirty two Sprague Dawley(SD) male rats were randomly divided into four groups: pseudo operation group, 45 d experimental group, 60 d experimental group and 90 d experimental group. Experimental varicocele was created by partial ligation of left renal vein. The apoptosis number of spermatogenic cells was measured by flow cytometry. Results: No marked apoptosis peak was observed in the control group, but it was observed in all the other groups, and the height of the peak increased with the duration of experiment. Conclusions: Varicocele can induce apoptosis of large numbers of spermatogenic cells, and the decrease of spermatogenic cells. This may be the mechanism of varicocele causing male sterility.
出处
《中华男科学杂志》
CAS
CSCD
2003年第7期507-509,511,共4页
National Journal of Andrology
基金
湖北省教育厅基金资助 (2 0 0 1Z0 40 0 5 )
