粉防己碱逆转P-gp介导的膀胱肿瘤多药耐药的实验研究

REVERSION OF P-GP MEDIATED MDR OF HUMAN BLADDER CACINOMA WITH TETRANDRINE IN VITRO

目的 观察粉防己碱 (Tetrandrine)对多药耐受糖蛋白 (P - glycoprotein ,P - gp)介导的膀胱癌细胞多药耐药 (MDR)的逆转。方法 MTT法观察MDR的逆转 ,流式细胞仪检测细胞内罗丹明 - 12 3浓度。结果 粉防己碱组与对照组对敏感株的细胞毒作用无显著差异 (P >0 .0 5 ) ;耐药株对粉防己碱组的敏感性是对照组的 4 .6倍 (P <0 .0 5 ) ,粉防己碱明显抑制了细胞内Rh - 12 3的外排 (P <0 .0 5 )。结论 粉防己碱可有效逆转P - gp介导的MDR。

Objective:To investigate reversion of P-gp mediated multidrug resistance of human bladder carcinoma cell lines with Tetrandrine in vitro.Methods:Cytotoxicity of Dox , Dox plus Tetrandrine were determined by measuring the inhibition of cell growth using the MTT assay, the amount of intracellular rhodamine-123 accumulation was determined by flow cytometry.Results:In the sensitive lines, the toxicity of Tetrandrine plus Dox paralleled that of l)ox, the MDR lines were 4. 6 times more sensitive to Dox plus Tetrandrine than to Dox, the increased sensitivity of MDR cells to Dox plus Tetrandrine was accounted for by inhibition of P-gp function.Conclusions:Tetrandrine was effective to overcome P-gp mediated MDR of human bladder cacinoma.