儿童脑损伤血清S-100B、神经元特异性烯醇化酶检测的意义

Significance of measurement for S-100B and neuron specific enolase in serum of head injury children

目的 研究不同程度脑损伤儿童血清S 10 0B、神经元特异性烯醇化酶 (NSE)浓度及其与格拉斯格昏迷评分法 (GCS)评分的相关性。方法 选择 2 0 0 1年 4月～ 2 0 0 2年 1月我院收治脑损伤后 2 4h内患儿 36例 ,按GCS评分标准将其分为重度脑损伤组 (组 1) 18例 ,GCS <8分 ;轻度脑损伤组 (组 2 ) 18例 ,GCS >12分 ;健康对照组 16例。脑损伤组于伤后 12～ 2 4h内均采集脑损伤组、健康对照组肘静脉血 4ml。分别用免疫荧光法和双抗体夹心免疫荧光法测定NSE及S 10 0B浓度。 6 2 0nm的酶标仪检测吸光度 ,通过标准曲线计算血清S 10 0B、NSE浓度。结果 组 1血清S 10 0B为 1.0 5± 0 .5 2 μg/L ,NSE为 6 9.13± 37.86 μg/L ;组 2S 10 0B 0 .4 7± 0 .2 1μg/L ,NSE为 2 6 .14± 13.4 6 μg/L ;对照组血清S 10 0B血清 0 .19± 0 .0 9μg/L ,NSE为 14 .4 3± 7.70 μg/L。S 10 0B组 1与组 2比较t=4 .5 7 P <0 .0 1;NSE组 1与组 2比较t =4 .5 4 P <0 .0 1;组 2与对照组比较S 10 0B :t=4 .72 P <0 .0 1,NSE :t=3.0 6 P <0 .0 1;组 1与对照组比较S 10 0B :t=6 .5 7 P =0 .0 0 ,NSE :t=5 .6 7 P =0 .0 0。S 10 0B与GCS评分的相关系数r =- 0 .86 9,F =12 6 .6 6 P =0 .0 0 0 ;r =- 0 .86 9,t =- 11.2 2 5 P =0 .

Objective To study the significance of measurement for S 100B and neuron specific enolase(NSE) in serum of children head injury and relation with GCS.Methods Blood samples were taken shortly after the trauma(12 24 hour)on18 case severe head injury patients(GCS<8)and 18 case minor head injury patients(GCS>12).Blood was allowed to clot and after centrifugation for 30 mins(3000 g,4 mins)serum was stored at-30 ℃for later analysis.Serum protein S 100B and NSE were analyzed by use of immunoluminometric assays.In 16 case healthy children these markers were also measure.S 100B and NSE concentrations in serum of patients and controls were compared.Results The differences of serum S 100B and NSE in each group(group1,group2,and controls)are significance when compared each other.The values of mean serum S 100B:1.05±0.52 μg/L,NSE:69.13±37.86 μg/L,group 2 S 100B: 0.466 ±0.21 μg/L,NSE:26.144±13.46 μg/L,controls,S 100B:0.191±0.09 μg/L,NSE 14.43±7.703 μg/L,compared between groupl and group 2 S 100B( t =4.566 P <0.01),NSE( t =4.538 P <0.01);group 2 and control S 100B( t =4.724 P < 0.01 );NSE( t =3.059 P <0.01)groupl and sciences,control S 100B( t =6.7 P =0.01);NSE( t =5.665 P =0.00).Conclusions S 100B concentration was higher in severe head injury patients than that of in minor head injury patients.Median NSE concentration was higher in patients than that of in controls and severe head injury higher in patients than that of minor head injury patients.S 100B and NSE are a useful marker for brain damage and neuron damage in severe and minor head injury.