摘要
目的 :探讨二种镍化合物诱发细胞恶变过程中端粒酶活性的变化。方法 :二种镍化合物转化细胞接种BALB/c裸鼠 ,应用端粒重复序列扩增法(telomericrepeatamplificationprotocol,TRAP)检测转化细胞和肿瘤细胞的端粒酶活性。 结果 :结晶型硫化镍和氯化镍诱发的BALB/c-3T3恶性转化细胞的端粒酶活性均表现为阳性。结论 :镍化合物诱发的细胞恶性转化涉及了端粒酶活性改变 。
Purpose: To explore the telomerase activity in the malignant process of BALB/c-3T3 cells induced by crystalline nickel sulfide, nickel chloride. Methods: TRAP-sliver staining assay was used to detect the telomerase activity. Results: Telomerase activity in BALB/c-3T3 cells transformed by nickel sulfide and nickel chloride were all positive. Conclusion: The positive expression of telomerase activity in the cell transformation might be related to the nickel carcinogenesis.[
出处
《癌变．畸变．突变》
CAS
CSCD
2003年第4期193-195,共3页
Carcinogenesis,Teratogenesis & Mutagenesis
基金
国家自然科学基金资助(No:39170651)
关键词
镍化合物
转化细胞
肿瘤细胞
端粒酶
nickel compounds
transformed cells
tumor cells
telomerase activity
