摘要
AIM: To study the effect of hydroxyapatite (HAP) nanoparticleson human hepatoma cell line BEL-7402 in vitro.METHODS: The human hepatoma cell line BEL-7402 wascultured and treated with HAP nanoparticles at variousconcentrations. Growth suppression was detected with MTTcolorimetric assay, cell apoptotic alterations were evaluatedby cytochemical staining (Hoechst 33258), transmissionelectron microscopy (TEM), and flow cytometry (FCM).RESULTS: HAP nanoparticles inhibited the growth ofhepatoma cells in a dose-dependent manner, with IC50 valuesof 29.30 mg/L. Treated with 50-200 mg/L HAP nanoparticlesfor 48 h, BEL-7402 cells apoptosis with nuclear chromatincondensation and fragmentation as well as cell shrinkageand the formation of apoptotic bodies were observed undercytochemical staining and transmission electron microscopy.FCM analysis showed hypodiploid peaks on histogram, theapoptotic rates at the concentrations of 50, 75, 100, 150and 200 mg/L of HAP nanoparticles were 20.35±2.23%,25.35±1.92%, 29.34±4.61%, 44.92±3.78 % and53.64±3.49%, respectively, which were all significantlyhigher than that of control group 2.23±0.14%. There wasa significant correlation between HAP nanoparticleconcentration and apoptotic rate (r=0.994, P＜0.01).CONCLUSION: HAP nanoparticles not only inhibitproliferation but also induce apoptosis of human hepatoma cell line BEL-7402 in vitro.
AIM:To study the effect of hydroxyapatite(HAP)nanoparticles on human hepatoma cell line BEL-7402 in vitro. METHODS:The human hepatoma cell line BEL-7402 was cultured and treated with HAP nanoparticles at various concentrations.Growth suppression was detected with MTT colorimetric assay,cell apoptotic alterations were evaluated by cytochernical staining(Hoechst 33258),transmission electron microscopy(TEM),and flow cytometry(FCM). RESULTS:HAP nanoparticles inhibited the growth of hepatoma cells in a dose-dependent manner,with IC_(50)values of 29.30 mg/L.Treated with 50-200 mg/L HAP nanoparticles for 48 h,BEL-7402 cells apoptosis with nuclear chromatin condensation and fragmentation as well as cell shrinkage and the formation of apoptotic bodies were observed under cytochemical staining and transmission electron microscopy. FCM analysis showed hypodiploid peaks on histogram,the apoptotic rates at the concentrations of 50,75,100,150 and 200 mg/L of HAP nanoparticles were 20.35±2.23 %, 25.35±1.92 %,29.34±4.61%,44.92±3.78 % and 53.64±3.49 %,respectively,which were all significantly higher than that of control group 2.23±0.14 %.There was a significant correlation between HAP nanoparticle concentration and apoptotic rate(r=0.994,P<0.01). CONCLUSION:HAP nanoparticles not only inhibit proliferation but also induce apoptosis of human hepatoma cell line BEL-7402 in vitro.
