摘要
AIM: To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region, 1p36.33-p36.23, in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes.METHODS: Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods, and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals, all with Hah ethical origin, to perform this casecontrol study. The haplotypes with significant difference in the gene (s) were further analyzed.RFSULTS: Among the 23 SNPs, 8 were found to be common in Chinese Han population. Allele frequency of one SNP,rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P＜0.05). Furthermore, haplotypes at five SNP sites of PRKCZ gene were identified.CONCLUSION: PRKCZgene may be associated with type 2 diabetes in Hah population in North China. The haplotypes at five SNP sites in this gene may be responsible for this association.
AIM:To identify the susceptible gene (s) for type 2 diabetes in the prevousely mapped region,1p36.33-p36.23,in Han population of North China using single nucleotide polymorphisms (SNPs) and to analyze the haplotypes of the gene (s) related to type 2 diabetes. METHODS:Twenty three SNPs located in 10 candidate genes in the mapped region were chosen from public SNP domains with bioinformatic methods,and the single base extension (SBE) method was used to genotype the loci for 192 sporadic type 2 diabetes patients and 172 normal individuals,all with Han ethical origin,to perform this case- control study.The haplotypes with significant difference in the gene (s) were further analyzed. RESULTS:Among the 23 SNPs,8 were found to be common in Chinese Han population.Allele frequency of one SNP, rs436045 in the protein kinase C/ζgene (PRKCZ) was statistically different between the case and control groups (P<0.05).Furthermore,haplotypes at five SNP sites of PRKCZ gene were identified. CONCLUSION:PRKCZ gene may be associated with type 2 diabetes in Han population in North China.The haplotypes at five SNP sites in this gene may be responsible for this association.
基金
the National Natural Science Foundation of China, No.39896200,No30170441
the National High Technology Research and Development Program,No.2001AA221161,No.2002BA711A05,No.2002BA71 IA10-02
The National Program for Key Basic Research Projects,No.G1998051016
the Natural Science Foundation of Beijing,No.7002026
