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嗅内衬细胞的培养 被引量：2

The culture of olfactory ensheathing cell
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摘要 目的 :探索一种简单的培养纯化嗅内衬细胞的方法。方法 :取新生 2 0 d的大鼠嗅球表层 ,以阿糖胞苷和牛垂体提取液分别作用 ,应用差速贴壁分选方法纯化培养细胞 ,以P75NGFR抗体鉴定。结果 :取得了较高纯度的嗅内衬细胞。结论 :应用阿糖胞苷和牛垂体提取液 ,联合差速贴壁分选法是有效可行的获取高纯度嗅内衬细胞的方法。
作者 王春生 汪德芬 郑谨 党晓谦 王坤正 陈君长
机构地区 西安交通大学第二医院骨科 西安市中医医院急诊科 西安交通大学免疫病理研究所
出处 《陕西医学杂志》 CAS 北大核心 2003年第9期771-773,F004,共4页 Shaanxi Medical Journal
关键词 嗅内衬细胞 细胞培养 大鼠 纯度 脊髓损伤 细胞移植
分类号 R651.2 [医药卫生—外科学]
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参考文献9

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同被引文献19

  • 1Regerio F, de Souza Queiroz L, Teixeira SA, et al. Neuroprotective action of melatonin on neonatal rat motoneurons after sciatic nerve transection. Brain Res, 2002, 926 (1-2): 33-41.
  • 2Chambers CB, Muma NA. Tau mRNA isoforms following sciatic nerve axotomy with and without regeneration. Brain Res Mol Brain Res, 1997, 48(1): 115-124.
  • 3Harding DI, Greensmith L, Anderson PN, et al. Motorneurons innervating partially denervated rat hindlimb muscle remain susceptible to axotomy-induced cell death. Neuroscience, 1998, 86(1): 291-299.
  • 4Grossman SD, Rosenberg LJ, Wrathall JR. Temporal-spatial pattern of acute neuronal and glial loss after spinal cord contusion. Exp Neurol, 2001, 168(2): 273-282.
  • 5Decherchi P, Vuillon-Cacciutolo G, Darques JL, et al. Changes in afferent activities from tibialis anterior muscle after nerve repair by self-anastomosis. Muscle Nerve, 2001, 24(1):59-68.
  • 6Billington L,Carlson BM. The recovery of long-term denervated rat muscles after Marcaine treatment and grafting. J Neurol Sci, 1996, 144(1-2): 147-155.
  • 7Ohara S,Tantuwaya V, DiStefano PS, et al. Exogenous NT-3 mitigates the transganglionic neuropeptide Y response to sciatic nerve injury. Brain Res, 1995, 699(1): 143-148.
  • 8Fabio R,Luciano de SQ,Simone AT,et al.Neuroprotective action of melatonin on neonatal rat motoneurons after sciatic nerve transection.Brain Research 2002;926(1-2):33-41
  • 9Christopher BC,Nancy AM.Tau mRNA isoforms following sciatic nerve axotomy with and without regeneration.Molecular Brain research 1997;48(1):115-24
  • 10D I Harding,L Greensmith,P N Anderson,et al.Motoneurons innervating partially denervated rat hindlimb muscles remain susceptible to axotomy-induced cell death.Neuroscience 1998;86(1):291-99

引证文献2

二级引证文献5

陕西医学杂志
2003年 第9期

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