摘要
目的 探讨Th1/Th2类细胞因子基因在人脑胶质瘤中的表达情况,以及它们在脑胶质瘤发生及发展中的作用。方法 以IL-2、IFNγ代表Th1类细胞因子,IL-4、IL-6及IL-10代表Th2类细胞因子,采用逆转录聚合酶链反应方法(RT-PCR)检测Th1/Th2类细胞因子基因在脑胶质瘤细胞、肿瘤浸润淋巴细胞及细胞株中的表达情况。结果 人脑胶质瘤细胞、肿瘤浸润淋巴细胞及脑胶质瘤细胞株均呈现明显的Th2类细胞因子基因优势表达,而正常人脑组织中则无这种表达趋势。结论Th2类细胞因子在人脑胶质瘤中的优势表达可能是导致脑胶质瘤患者细胞免疫功能低下的原因之一,有可能在脑胶质瘤的发生及发展中起一定作用。
Objective To explore the expression of Th1/Th2 cytokines gene in human gliomas and its action in occurring and developing of human gliomas. Method Using IL-2 and IFN γ as Th1 type cytokines, IL-4, IL-6 and IL-10 as Th2 type cytokines, the gene expression of Th1/Th2 eytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines were detected by RT-PCR. Results There was predominant expression of Th2 type cytokines in human glioma cells, glioma infiltrating lymphocytes and glioma cell lines, but there was no such expression in normal brain tissue. Conclusion It suggest that there is a relationship between the Th2 type cytokines expression in human gliomas and the immunosuppressive status of human glioma patients. The predominant expression of Th2 type cytokines may play an important role in occurring and developing of human gliomas.
出处
《中华神经外科杂志》
CSCD
北大核心
2003年第5期348-351,共4页
Chinese Journal of Neurosurgery
基金
国家自然科学基会资助项目(项目批准号30271335)
